Difference between revisions of "Part:BBa K3110036:Design"
 
Line 6: Line 6:
  
 
===Source===
 
===Source===
−
Strong promoter -Strong RBS-lldP flank and lldD flank-terminator were synthesized by IDT. <br>
+
Medium promoter - Weak RBS-lldP flank and lldD flank-terminator were synthesized by IDT. <br>
 
lldP+lldR+lldD was amplified from the genome.
 
lldP+lldR+lldD was amplified from the genome.
 
===References===
 
===References===

Latest revision as of 17:26, 21 October 2019


Medium Promoter Weak RBS lldP+lldR+lldD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 327
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3649
    Illegal AgeI site found at 463
    Illegal AgeI site found at 926
    Illegal AgeI site found at 1364
    Illegal AgeI site found at 1523
    Illegal AgeI site found at 1535
    Illegal AgeI site found at 2281
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3086

Source

Medium promoter - Weak RBS-lldP flank and lldD flank-terminator were synthesized by IDT.
lldP+lldR+lldD was amplified from the genome.

References

iGEM ETH zurich 2015
iGEM NUS Singapore 2016
Aguilera, L., Campos, E., Gimenez, R., Badia, J., Aguilar, J., & Baldoma, L. (2008). Dual Role of LldR in Regulation of the lldPRD Operon, Involved in L-Lactate Metabolism in Escherichia coli. Journal of Bacteriology, 190(8), 2997–3005. doi:10.1128/jb.02013-07