Difference between revisions of "Part:BBa K3285700"

Revision as of 17:12, 21 October 2019

Lead Sequestration Protein with RBS

pbrD is a protein that sequesters lead. It is present downstream of a strong RBS (BBa_B0034) and binds to lead ions in the cytoplasm and decrease its concentration.

Usage and Biology

This is an intermediate part which can be incorporated under a promoter. The pbrD protein is known to sequester lead ions in the cytoplasm of the bacterial cell.

When put under a lead responsive promoter, the part is expected to show dosage dependent sequestration properties.

Construction

File:PbrD Gel.jpeg

The part was artificially synthesised with corresponding primers that have overlap with GFP sequence. This allows it to be put downstream of the lead dependent biosensor <a href="https://parts.igem.org/Part:BBa_K1758333">(BBa_K1758333)</a>. This part has been synthesised with a strong Ribosome Binding Site <a href="https://parts.igem.org/Part:BBa_B0034">(BBa_B00340)</a> upstream of the coding sequence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 532
1000
COMPATIBLE WITH RFC[1000]

@@ Line 13: / Line 13: @@
 ===Construction===
-{|align="left"
+{|align="right"
 ! [[file:pbrD_Gel.jpeg|200px|]]
 |}
-The part was artificially synthesised with corresponding primers that have overlap with GFP sequence. This allows it to be put downstream of the lead dependent biosensor (<a href="https://parts.igem.org/Part:BBa_K1758333">BBa_K1758333</a>). This part has been synthesised with a strong Ribosome Binding Site (<a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>) upstream of the coding sequence.
+The part was artificially synthesised with corresponding primers that have overlap with GFP sequence. This allows it to be put downstream of the lead dependent biosensor <a href="https://parts.igem.org/Part:BBa_K1758333">(BBa_K1758333)</a>. This part has been synthesised with a strong Ribosome Binding Site <a href="https://parts.igem.org/Part:BBa_B0034">(BBa_B00340)</a> upstream of the coding sequence.
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