Difference between revisions of "Part:BBa K3098005:Experience"
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BC pellets formed by ''G. xylinus'' on silk surface | BC pellets formed by ''G. xylinus'' on silk surface | ||
Latest revision as of 17:10, 21 October 2019
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K3098005
We have used this part to produce Bacteria Cellulose (BC) in E. coli BL21(DE3) successfully. We also have compared the yield of BC in engineered E. coli BL21(DE3) and the source microbe Glucanacetobacter xylinus. We measure the concentration of cellulose in media by anthrone method. Though lower than G. xylinus, the yield of BC in engineered E. coli is also significantly different with the negative control.
NOTE: We got the data above on the condition that uninduced E.coli and E.coli media was diluted to 1/5 and G. xylinus medium was diluted to 1/91.
Furthermore, according to our aim to reinforce silk by BC, we observed the BC secreted by engineered E. coli and G. xylinus on silk surface by electron microscope.
BC secreted by engineered E. coli conneceting between silk fiborins
engineered E. coli on silk surface
BC secreted by G. xylinus on silk surface
BC pellets formed by G. xylinus on silk surface
User Reviews
UNIQ1ba697e501262d44-partinfo-00000000-QINU UNIQ1ba697e501262d44-partinfo-00000001-QINU