Difference between revisions of "Part:BBa K2940001"
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<partinfo>BBa_K2940001 short</partinfo> | <partinfo>BBa_K2940001 short</partinfo> | ||
| − | The PelB leader sequence was joined to Bpul laccase to enable exportation of the enzyme into the periplasmic space. Both parts were isolated from the iGEM kit via Q5 PCR and joined together using Gibson Assembly. | + | The PelB leader sequence was joined to Bpul laccase to enable exportation of the enzyme into the periplasmic space. Both parts were isolated from the iGEM kit via Q5 PCR and joined together using Gibson Assembly. Not only was exporting Bpul outside of the cell vital for our overall project, it also allowed us to assess the activity of Bpul without the need for expensive and time consuming purification steps. |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | ===Characterisation=== | |
[[Image:T--Edinburgh OG--SDS.png|frame|]] | [[Image:T--Edinburgh OG--SDS.png|frame|]] | ||
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Revision as of 17:09, 21 October 2019
PelB and Bpul
The PelB leader sequence was joined to Bpul laccase to enable exportation of the enzyme into the periplasmic space. Both parts were isolated from the iGEM kit via Q5 PCR and joined together using Gibson Assembly. Not only was exporting Bpul outside of the cell vital for our overall project, it also allowed us to assess the activity of Bpul without the need for expensive and time consuming purification steps.
Usage and Biology
Characterisation
