Difference between revisions of "Part:BBa K3098005:Experience"

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Furthermore, according to our aim to reinforce silk by BC, we observed the BC secreted by engineered ''E. coli'' and ''G. xylinus'' on silk surface by electron microscope.
 
Furthermore, according to our aim to reinforce silk by BC, we observed the BC secreted by engineered ''E. coli'' and ''G. xylinus'' on silk surface by electron microscope.
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[[File:Ecoli_m004.png|600px|center]]
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<center>
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BC secreted by engineered ''E. coli'' conneceting between silk fiborins
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</center>
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[[File:Ecoli_m009.png|600px|center]]
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<center>
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engineered ''E. coli'' on silk surface
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</center>
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[[File:Gxylinus_005.png|600px|center]]
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<center>
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BC secreted by ''G. xylinus'' on silk surface
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</center>
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[[File:Gxylinus_009.png|600px|center]]
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<center>
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BC pellets formed by ''G. xylinus'' on silk surface
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</center>
  
 
===User Reviews===
 
===User Reviews===

Revision as of 17:06, 21 October 2019


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3098005

We have used this part to produce Bacteria Cellulose (BC) in E. coli BL21(DE3) successfully. We also have compared the yield of BC in engineered E. coli BL21(DE3) and the source microbe Glucanacetobacter xylinus. We measure the concentration of cellulose in media by anthrone method. Though lower than G. xylinus, the yield of BC in engineered E. coli is also significantly different with the negative control.

Yield of BC.png

NOTE: We got the data above on the condition that uninduced E.coli and E.coli media was diluted to 1/5 and G. xylinus medium was diluted to 1/91.

Furthermore, according to our aim to reinforce silk by BC, we observed the BC secreted by engineered E. coli and G. xylinus on silk surface by electron microscope.


Ecoli m004.png

BC secreted by engineered E. coli conneceting between silk fiborins



Ecoli m009.png

engineered E. coli on silk surface


600px

BC secreted by G. xylinus on silk surface


600px

BC pellets formed by G. xylinus on silk surface

User Reviews

UNIQ8455938de50b719b-partinfo-00000000-QINU UNIQ8455938de50b719b-partinfo-00000001-QINU