Difference between revisions of "Part:BBa K2753045"
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A promoter | A promoter | ||
| + | ==Contribution: BNDS_China 2019== | ||
| + | <div> | ||
| + | <b>Group: BNDS_China iGEM 2019</b> | ||
| + | <br> | ||
| + | <b>Author: Wanji Li</b> | ||
| + | <br> | ||
| + | <b>Introduction & Design:</b> | ||
| + | <br> | ||
| + | |||
| + | In order to determine the strength of this promoter at different temperature and glucose concentration, we constructed a plasmid with the expression of GFP as an indicator downstream the TEF1 promoter and measured its intensity of fluorescence. Additionally， we also constructed a plasmid with GFP only as a negative control. | ||
| + | |||
| + | <html> | ||
| + | <div style="text-align:center";> | ||
| + | <br> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/9/9d/T--BNDS_China--pTEF1_characterization.png></br> | ||
| + | <p style="width:600px; margin-left:150px; margin-bottom:60px; | ||
| + | text-align:center | ||
| + | "><em><strong>FIGURE 1</strong>(A) the intensity of fluorescence under different concentration of glucose in 30 degree Celsius; (B) the intensity of fluorescence under different concentration of glucose in 32 degree Celsius; (C) the intensity of fluorescence under different concentration of glucose in 34 degree Celsius; (D) the intensity of fluorescence under different concentration of glucose in 37 degree Celsius</em> </p> | ||
| + | <br> | ||
| + | </div> | ||
| + | </html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 17:06, 21 October 2019
TEF1 promoter
A promoter
Contribution: BNDS_China 2019
Group: BNDS_China iGEM 2019
Author: Wanji Li
Introduction & Design:
In order to determine the strength of this promoter at different temperature and glucose concentration, we constructed a plasmid with the expression of GFP as an indicator downstream the TEF1 promoter and measured its intensity of fluorescence. Additionally， we also constructed a plasmid with GFP only as a negative control.
FIGURE 1(A) the intensity of fluorescence under different concentration of glucose in 30 degree Celsius; (B) the intensity of fluorescence under different concentration of glucose in 32 degree Celsius; (C) the intensity of fluorescence under different concentration of glucose in 34 degree Celsius; (D) the intensity of fluorescence under different concentration of glucose in 37 degree Celsius
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]