Difference between revisions of "Part:BBa K2972001:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The two ends of this sequence are highly similar to sigmaB. It may lead to the failure of PCR.
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Sigma F is derived from Strains B. subtilis subsp. subtilis wild-type (LMG 7135), and it’s a regulatory protein that is responsible for directing gene expression in the forespore compartment of developing cells of the spore-forming soil bacterium Bacillus subtilis.
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===Source===
 
===Source===

Revision as of 17:05, 21 October 2019


Sigma F


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101


Design Notes

Sigma F is derived from Strains B. subtilis subsp. subtilis wild-type (LMG 7135), and it’s a regulatory protein that is responsible for directing gene expression in the forespore compartment of developing cells of the spore-forming soil bacterium Bacillus subtilis.

Source

It come from Bacillus subtilis genomic sequence.

References