Difference between revisions of "Part:BBa K3229290"
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This is the putative promoter of microcystin toxin-producing proteins from Microcysis aeruginosa NIES-843. We looked up the promoter sequence in Ensemble Bacteria (http://bacteria.ensembl.org). We found many sequences that differ between strains, by the help of Geneious Prime we found the strain NIES-843 which has the most conservative sequence based on the consensus sequence we got. From the Microcystis promoter transcription can occur in both directions to synthesize the McyABC and McyDEFGHIJ proteins. These proteins are responsible for the nonribosomal peptide synthesis of the toxin, called microcystin. The presence of this toxin enhances the production of toxins in other cells via the phenomenon called autoinduction. For this reason, we could use this promoter in our microcystin detecting device. | This is the putative promoter of microcystin toxin-producing proteins from Microcysis aeruginosa NIES-843. We looked up the promoter sequence in Ensemble Bacteria (http://bacteria.ensembl.org). We found many sequences that differ between strains, by the help of Geneious Prime we found the strain NIES-843 which has the most conservative sequence based on the consensus sequence we got. From the Microcystis promoter transcription can occur in both directions to synthesize the McyABC and McyDEFGHIJ proteins. These proteins are responsible for the nonribosomal peptide synthesis of the toxin, called microcystin. The presence of this toxin enhances the production of toxins in other cells via the phenomenon called autoinduction. For this reason, we could use this promoter in our microcystin detecting device. | ||
| + | https://static.igem.org/mediawiki/parts/7/73/T--SZTA_Szeged_HU--Originalsequence.png | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 16:59, 21 October 2019
McyA/D bidirectional promoter
This is the putative promoter of microcystin toxin-producing proteins from Microcysis aeruginosa NIES-843. We looked up the promoter sequence in Ensemble Bacteria (http://bacteria.ensembl.org). We found many sequences that differ between strains, by the help of Geneious Prime we found the strain NIES-843 which has the most conservative sequence based on the consensus sequence we got. From the Microcystis promoter transcription can occur in both directions to synthesize the McyABC and McyDEFGHIJ proteins. These proteins are responsible for the nonribosomal peptide synthesis of the toxin, called microcystin. The presence of this toxin enhances the production of toxins in other cells via the phenomenon called autoinduction. For this reason, we could use this promoter in our microcystin detecting device.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 578
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]