Difference between revisions of "Part:BBa K2940001"
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The PelB leader sequence was joined to Bpul laccase to enable exportation of the enzyme into the periplasmic space.  Both parts were isolated from the iGEM kit via Q5 PCR and joined together using Gibson Assembly.  
 
The PelB leader sequence was joined to Bpul laccase to enable exportation of the enzyme into the periplasmic space.  Both parts were isolated from the iGEM kit via Q5 PCR and joined together using Gibson Assembly.  
  
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<h2>Usage and Biology</h2>
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===Usage and Biology===
  
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<b> Cultivation and SDS Page </b>
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'''Cultivation and SDS Page'''
  
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[[Image:T--Edinburgh OG--SDS.png|frame|caption]]
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[[Image:T--Edinburgh OG--SDS.png|200px|]]
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 16:52, 21 October 2019


PelB and Bpul

The PelB leader sequence was joined to Bpul laccase to enable exportation of the enzyme into the periplasmic space. Both parts were isolated from the iGEM kit via Q5 PCR and joined together using Gibson Assembly.

Usage and Biology

Cultivation and SDS Page

T--Edinburgh OG--SDS.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 195
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 562
    Illegal NgoMIV site found at 1030
    Illegal AgeI site found at 318
    Illegal AgeI site found at 1195
  • 1000
    COMPATIBLE WITH RFC[1000]