Difference between revisions of "Part:BBa K2992002"

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===Characterisation===
 
===Characterisation===
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This is a basic part that we used in many experiments to produce the sigma factor to induce the expression of acetone, GusA or FAST.
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This basic part was used in many experiments to produce the sigma factor to induce the expression of acetone, GusA or FAST.
 
We made three <i>BotR</i>-expression modules comprising of the <i>BotR</i> sigma factor gene under the control of the native <i>BotR</i> promotor,an inducible system and one with no promotor. See the[https://2019.igem.org/Team:Nottingham/Results results page] for more information.  
 
We made three <i>BotR</i>-expression modules comprising of the <i>BotR</i> sigma factor gene under the control of the native <i>BotR</i> promotor,an inducible system and one with no promotor. See the[https://2019.igem.org/Team:Nottingham/Results results page] for more information.  
  

Revision as of 16:50, 21 October 2019


botR gene from C. botulinum

botR encodes a positive transcriptional regulator of C. botulinum neurotoxin production which functions as an alternative sigma factor.

Usage and Biology

BotR regulates the the expression of boutlinum neruotoxin and non-toxic associated proteins during the late exponential and early stationary growth phases. We have integrated botR into the genome of C. sporogenes to generate a reporter strain to validate the concept of using volatile reporters to predict the production of botulinum neurotoxin following food manufacturing processes.

Characterisation

This basic part was used in many experiments to produce the sigma factor to induce the expression of acetone, GusA or FAST. We made three BotR-expression modules comprising of the BotR sigma factor gene under the control of the native BotR promotor,an inducible system and one with no promotor. See theresults page for more information.


T--Nottingham--Basic4.png
T--Nottingham--Basic3.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Raffestin, S., Dupuy, B., Marvaud, J. and Popoff, M. (2004). BotR/A and TetR are alternative RNA polymerase sigma factors controlling the expression of the neurotoxin and associated protein genes in Clostridium botulinum type A and Clostridium tetani. Molecular Microbiology, 55(1), pp.235-249.

Zhang, Z., Korkeala, H., Dahlsten, E., Sahala, E., Heap, J., Minton, N. and Lindström, M. (2013). Two-Component Signal Transduction System CBO0787/CBO0786 Represses Transcription from Botulinum Neurotoxin Promoters in Clostridium botulinum ATCC 3502. PLoS Pathogens, 9(3), p.e1003252.