Difference between revisions of "Part:BBa K3053069"
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This part regulates our system when it is present in a non antibiotic medium where Cro is not expressed. | This part regulates our system when it is present in a non antibiotic medium where Cro is not expressed. | ||
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here--> |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | <p>The part is designed in such a way that it is complementary to the RBS (BBa_K3503001). This way, when no ampicillin resistance gene is present (<i>bla</i>) it forms a double stem loop with the RBS and inhibits the expression of the Cro gene which induces the lysogenic cycle of the phage. | |
| + | </p> | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'><b>Sequence and Features</b></span> | <span class='h3bb'><b>Sequence and Features</b></span> | ||
Latest revision as of 16:38, 21 October 2019
Reverse Complement
This part regulates our system when it is present in a non antibiotic medium where Cro is not expressed.
Usage and Biology
The part is designed in such a way that it is complementary to the RBS (BBa_K3503001). This way, when no ampicillin resistance gene is present (bla) it forms a double stem loop with the RBS and inhibits the expression of the Cro gene which induces the lysogenic cycle of the phage.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]