Difference between revisions of "Part:BBa K3225015"

 
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<partinfo>BBa_K3225015 short</partinfo>
 
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This device is designed for detect quorum sensing molecule&#65288;AHL&#65289;of Erwinia Carotovora in cell-free system. It consists of LuxR(quorum sensing transcriptional factor, BBa_C0062) drived by a constitutive promoter J23101 and LacZ(BBa_I732017) as a repoter drived by the binding promoter of LuxR(pLux, BBa_R0062).
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This gene circuit is designed to detect N-(3-oxohexanoyl)-L-homoserine lactone (OHHL https://2019.igem.org/wiki/images/0/0c/T--iBowu-China--DesignOHHL.png ，quorum sensing signal molecular of E.carotovora). It consists of LuxR(quorum sensing transcriptional factor, BBa_C0062) drove by a constitutive promoter J23101 and LacZ(BBa_I732017) as a repoter drove by the binding promoter of LuxR(pLux, BBa_R0062).  
  
  
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<partinfo>BBa_K3225015 parameters</partinfo>
 
<partinfo>BBa_K3225015 parameters</partinfo>
 
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==Results==
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We placed the physical gene circuits to papers by freeze-drying technology. The report is LacZ which is β-galactosidase which can cleave the yellow substrate to purple. Among the series of AHL concentrations induction, it showed that the detection limit was lower than 1 nM. Moreover, there is a significant visible output with 10 nm AHL treatment (Fig. 1).
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Figure 1. The detection limit and detection time of quorum sensing based testing strips.
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<img style="width:200px;"src="https://2019.igem.org/wiki/images/0/09/T--iBowu-China--jin.jpg">

Revision as of 16:38, 21 October 2019


J23101-LuxR-pLux-LacZ

This gene circuit is designed to detect N-(3-oxohexanoyl)-L-homoserine lactone (OHHL T--iBowu-China--DesignOHHL.png ，quorum sensing signal molecular of E.carotovora). It consists of LuxR(quorum sensing transcriptional factor, BBa_C0062) drove by a constitutive promoter J23101 and LacZ(BBa_I732017) as a repoter drove by the binding promoter of LuxR(pLux, BBa_R0062).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Results

We placed the physical gene circuits to papers by freeze-drying technology. The report is LacZ which is β-galactosidase which can cleave the yellow substrate to purple. Among the series of AHL concentrations induction, it showed that the detection limit was lower than 1 nM. Moreover, there is a significant visible output with 10 nm AHL treatment (Fig. 1).

Figure 1. The detection limit and detection time of quorum sensing based testing strips.

<img style="width:200px;"src="T--iBowu-China--jin.jpg">