Difference between revisions of "Part:BBa K2949012"

Revision as of 16:35, 21 October 2019

Carbonic Anhydrase 2(L203K)-N-LCTASR

we constructed this new biobrick by connecting the N-terminal of the mutant human carbonic anhydrase 2 [CA2(L203K)] (BBa_K2547004) with the six-residue sulfatase submotif (LCTPSR). Formylglycine generating enzyme can selectively recognize and oxidize the cysteine residues in the sulfatase subunit of the protein terminal to form formylglycine (FGly) residues containing aldehyde groups, which can be immobilized via forming covalent bond with amino functionalized carriers (Unisil 30-100 NH2) through the Schiff base reaction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2949012 short</partinfo>
-This part is the coding sequence (CDS) of the N-terminal modified Carbonic anhydrase 2(L203K)(BBa_K2547004).Formylglycine generating enzyme can selectively recognize and oxidize the cysteine residues in the sulfatase subunit of the protein terminal to form formylglycine (FGly) residues containing aldehyde groups.The terminal aldehyde group of CA2, is fixed to surface active carriers containing amino groups by covalent binding. This site-specific fixation avoids the chemical degeneration that may occur in common covalent fixation and has higher stability than physical adsorption.
+we constructed this new biobrick by connecting the N-terminal of the mutant human carbonic anhydrase 2 [CA2(L203K)] (BBa_K2547004) with the six-residue sulfatase submotif (LCTPSR). Formylglycine generating enzyme can selectively recognize and oxidize the cysteine residues in the sulfatase subunit of the protein terminal to form formylglycine (FGly) residues containing aldehyde groups, which can be immobilized via forming covalent bond with amino functionalized carriers (Unisil 30-100 NH2) through the Schiff base reaction.
 <!-- Add more about the biology of this part here