Difference between revisions of "Part:BBa K3046002:Design"

 
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===Design Notes===
 
===Design Notes===
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This part is created on the basis of the upstream region of the GlaA ORF (Systematic ORF name: An03g06550). It has been creating using the LEAP software, as described here: [insert link to wiki] 
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This part is optimised to be compatible with the MoClo standard, Mobius assembly standard and not to contain any SwaI cut sites.
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===Source===
 
===Source===
  
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This part is optimised to be compatible with [insert compatability data here]
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This part is created on the basis of the upstream region of the sonB ORF (Systematic ORF name: An04g05630) of many <i>aspergillus</i> genomes.It has been created using the proHMMoter software, as a part of the LEAP project, described in further detail here: https://2019.igem.org/Team:DTU-Denmark/Model
  
 
===References===
 
===References===

Revision as of 16:29, 21 October 2019


PLEAPsonB_1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 602
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 694
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 343
    Illegal BglII site found at 654
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 602
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 602
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is optimised to be compatible with the MoClo standard, Mobius assembly standard and not to contain any SwaI cut sites.


Source

This part is created on the basis of the upstream region of the sonB ORF (Systematic ORF name: An04g05630) of many aspergillus genomes.It has been created using the proHMMoter software, as a part of the LEAP project, described in further detail here: https://2019.igem.org/Team:DTU-Denmark/Model

References