Difference between revisions of "Part:BBa K2972004:Design"

 
 
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===Design Notes===
 
===Design Notes===
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...
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The identification of this gene in E. coli prokaryotic expression system is currently mainly accomplished by heterologous complementation detection.
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When designing primers, it is recommended that the first 6 bps of the 5' end cannot form a card issuance structure.
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===Source===
 
===Source===
  
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From...
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The sequence of this part is obtained from NCBI and it comes from an E.coli's genome which intergrated lycopene gene.
  
 
===References===
 
===References===

Latest revision as of 16:26, 21 October 2019


Phytoene desaturase (crtI)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 696
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 154
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 742
    Illegal SapI.rc site found at 190


Design Notes

The identification of this gene in E. coli prokaryotic expression system is currently mainly accomplished by heterologous complementation detection. When designing primers, it is recommended that the first 6 bps of the 5' end cannot form a card issuance structure.

Source

The sequence of this part is obtained from NCBI and it comes from an E.coli's genome which intergrated lycopene gene.

References