Difference between revisions of "Part:BBa K3002100"
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<partinfo>BBa_K3002100 short</partinfo>
 
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This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), cCA secretion signal (BBa_K3002007), Sp20HA-tag (BBa_K3002010) and the coding sequence of the mutant PETase, Mutate M, of the iGEM team TJUSLS China (BBa_K1921003) plus the MoClo connectors for positions B1-B2 (BBa_K3002302), B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304 and B5-B6 (BBa_K3002305).  
 
This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), cCA secretion signal (BBa_K3002007), Sp20HA-tag (BBa_K3002010) and the coding sequence of the mutant PETase, Mutate M, of the iGEM team TJUSLS China (BBa_K1921003) plus the MoClo connectors for positions B1-B2 (BBa_K3002302), B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304 and B5-B6 (BBa_K3002305).  
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Revision as of 16:26, 21 October 2019


Level 1 - Mutant PETase + cCA + SP20 HA (Phytobrick)

This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), cCA secretion signal (BBa_K3002007), Sp20HA-tag (BBa_K3002010) and the coding sequence of the mutant PETase, Mutate M, of the iGEM team TJUSLS China (BBa_K1921003) plus the MoClo connectors for positions B1-B2 (BBa_K3002302), B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304 and B5-B6 (BBa_K3002305).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1909
  • 1000
    COMPATIBLE WITH RFC[1000]