Difference between revisions of "Part:BBa K3224003"

Revision as of 16:20, 21 October 2019

BP100-(KH)₉

Cell-Penetrating Peptides (CPP) are short peptides that can penetrate the cell membrane and deliver a wide range of cargoes into the cell. This part is the coding sequence of our chimeric CPP formed with BP100 and the polycationic peptide (KH)9. It is proven to deliver proteins and nucleic acids into the plant and prokaryotic cells. The polycationic peptide is an efficient nucleic acid carrier and has the ability to promote buffering effect on the pre-lysosomal vesicle. The cargo being negatively charged interacts with the polycationic end of the peptide through ionic interactions, whereas the BP100 region mediates cellular entry through carpet-mechanism.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3224003 short</partinfo>
-Cell-Penetrating Peptides (CPP) are short peptides that can penetrate the cell membrane and deliver a wide range of cargoes into the cell.
+Cell-Penetrating Peptides (CPP) are short peptides that can penetrate the cell membrane and deliver a wide range of cargoes into the cell. This part is the coding sequence of our chimeric CPP formed with BP100 and the polycationic peptide (KH)9. It is proven to deliver proteins and nucleic acids into the plant and prokaryotic cells. The polycationic peptide is an efficient nucleic acid carrier and has the ability to promote buffering effect on the pre-lysosomal vesicle. The cargo being negatively charged interacts with the polycationic end of the peptide through ionic interactions, whereas the BP100 region mediates cellular entry through carpet-mechanism.
 <!-- Add more about the biology of this part here