Difference between revisions of "Part:BBa K3286118:Design"

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<li> V. K. Mutalik et al., “Precise and reliable gene expression via standard transcription and translation initiation elements,” Nat. Methods, vol. 10, no. 4, pp. 354–360, Apr. 2013. </li>
 
<li> V. K. Mutalik et al., “Precise and reliable gene expression via standard transcription and translation initiation elements,” Nat. Methods, vol. 10, no. 4, pp. 354–360, Apr. 2013. </li>
 
<li> E. K. Petutschnig, A. M. E. Jones, L. Serazetdinova, U. Lipka, and V. Lipka, “The Lysin Motif Receptor-like Kinase (LysM-RLK) CERK1 Is a Major Chitin-binding Protein in Arabidopsis thaliana and Subject to Chitin-induced Phosphorylation * □ S,” 2010. </li>
 
<li> E. K. Petutschnig, A. M. E. Jones, L. Serazetdinova, U. Lipka, and V. Lipka, “The Lysin Motif Receptor-like Kinase (LysM-RLK) CERK1 Is a Major Chitin-binding Protein in Arabidopsis thaliana and Subject to Chitin-induced Phosphorylation * □ S,” 2010. </li>
 +
<li> Nieuwkoop, T., Claassens, N. J., & van der Oost, J. (2019). Improved protein production and codon optimization analyses in Escherichia coli by bicistronic design. Microbial biotechnology, 12(1), 173-179.</li>

Latest revision as of 16:19, 21 October 2019


Expression cassette for 3LysM in E. coli BL21DE3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 701
    Illegal XhoI site found at 266
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

See individual parts

Source

see individual parts

References

  • V. K. Mutalik et al., “Precise and reliable gene expression via standard transcription and translation initiation elements,” Nat. Methods, vol. 10, no. 4, pp. 354–360, Apr. 2013.
  • E. K. Petutschnig, A. M. E. Jones, L. Serazetdinova, U. Lipka, and V. Lipka, “The Lysin Motif Receptor-like Kinase (LysM-RLK) CERK1 Is a Major Chitin-binding Protein in Arabidopsis thaliana and Subject to Chitin-induced Phosphorylation * □ S,” 2010.
  • Nieuwkoop, T., Claassens, N. J., & van der Oost, J. (2019). Improved protein production and codon optimization analyses in Escherichia coli by bicistronic design. Microbial biotechnology, 12(1), 173-179.