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Revision as of 16:15, 21 October 2019


mClover3 Fluorescent Protein, Codon Optimized for L. reuteri

mClover3 is a green fluorescent protein derivative which has been codon optimized for Lactobacillus reuteri 10023C, and may have uses in other Lactobacillus species.


Figure 1: mClover3 spectrum
Figure 2: - ldh promoter FI and OD600 time dependence - Blank corrected Fluorescence intensity and OD600 was plotted against time for ldh promoter. A large peak in OD600 can be observed, which could be an outliar due to random error in the instrument. Error bars represent Standard error of the mean. n=3



































Fluorescence wavelengths

Bajar et al report the following excitation and emission data for mClover3 -

  • Excitation max - 506nm
  • Emission max - 518nm


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterisation

This part was characterised in the composite part BBa_K3183101. Data for this part is shown below.