Difference between revisions of "Part:BBa K3110047:Design"

 
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===Source===
 
===Source===
  
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FimH 136KO-RPMrel was optained from Biobrick. Promoter+RBS was obtained as gBlock from IDT. These were joined together using SOEing (Spliced Overlap Extenstion) PCR.
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FimH 136KO-RPMrel was optained from Biobrick. Promoter+RBS and terminator were obtained as gBlock from IDT. These were joined together using SOEing (Spliced Overlap Extenstion) PCR.
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===References===
 
===References===

Revision as of 16:03, 21 October 2019


Promoter RBS FimH 136KO-RPMrel


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 549
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

FimH 136KO-RPMrel was optained from Biobrick. Promoter+RBS and terminator were obtained as gBlock from IDT. These were joined together using SOEing (Spliced Overlap Extenstion) PCR.

References