Difference between revisions of "Part:BBa K3098005"
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<partinfo>BBa_K3098005 short</partinfo>
 
<partinfo>BBa_K3098005 short</partinfo>
  
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Cellulose GxCesA and GxCesB, with pT5 promoter, two RBS and terminator.
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Cellulose Synthase GxCesA ([[Part:BBa_K3098001]]) and GxCesB([[Part:BBa_K3098002]]), with T5 promoter, two different Rbs and terminator.
  
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===Usage and Biology===
 
===Usage and Biology===
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Used to express bacteria cellulose synthase in E. coli. After adding IPTG to induce, the engineered bacteria will synthesize and secrete bacteria cellulose.
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K3098005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3098005 SequenceAndFeatures</partinfo>
  

Revision as of 16:00, 21 October 2019


Cellulose Synthase Operon with T5 promoter

Cellulose Synthase GxCesA (Part:BBa_K3098001) and GxCesB(Part:BBa_K3098002), with T5 promoter, two different Rbs and terminator.

Usage and Biology

Used to express bacteria cellulose synthase in E. coli. After adding IPTG to induce, the engineered bacteria will synthesize and secrete bacteria cellulose.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 817
    Illegal PstI site found at 2706
    Illegal PstI site found at 2715
    Illegal PstI site found at 3015
    Illegal PstI site found at 3555
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 817
    Illegal PstI site found at 2706
    Illegal PstI site found at 2715
    Illegal PstI site found at 3015
    Illegal PstI site found at 3555
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 655
    Illegal BglII site found at 1217
    Illegal BamHI site found at 4346
    Illegal XhoI site found at 307
    Illegal XhoI site found at 3729
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 817
    Illegal PstI site found at 2706
    Illegal PstI site found at 2715
    Illegal PstI site found at 3015
    Illegal PstI site found at 3555
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 817
    Illegal PstI site found at 2706
    Illegal PstI site found at 2715
    Illegal PstI site found at 3015
    Illegal PstI site found at 3555
    Illegal NgoMIV site found at 287
    Illegal AgeI site found at 2268
    Illegal AgeI site found at 4401
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4172
    Illegal BsaI.rc site found at 1792