Difference between revisions of "Part:BBa K3098005"
Line 3: | Line 3: | ||
<partinfo>BBa_K3098005 short</partinfo> | <partinfo>BBa_K3098005 short</partinfo> | ||
− | Cellulose GxCesA and GxCesB, with | + | Cellulose Synthase GxCesA ([[Part:BBa_K3098001]]) and GxCesB([[Part:BBa_K3098002]]), with T5 promoter, two different Rbs and terminator. |
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | Used to express bacteria cellulose synthase in E. coli. After adding IPTG to induce, the engineered bacteria will synthesize and secrete bacteria cellulose. | ||
<!-- --> | <!-- --> | ||
− | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'>'''Sequence and Features'''</span> |
<partinfo>BBa_K3098005 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3098005 SequenceAndFeatures</partinfo> | ||
Revision as of 16:00, 21 October 2019
Cellulose Synthase Operon with T5 promoter
Cellulose Synthase GxCesA (Part:BBa_K3098001) and GxCesB(Part:BBa_K3098002), with T5 promoter, two different Rbs and terminator.
Usage and Biology
Used to express bacteria cellulose synthase in E. coli. After adding IPTG to induce, the engineered bacteria will synthesize and secrete bacteria cellulose.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 655
Illegal PstI site found at 817
Illegal PstI site found at 2706
Illegal PstI site found at 2715
Illegal PstI site found at 3015
Illegal PstI site found at 3555 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 655
Illegal PstI site found at 817
Illegal PstI site found at 2706
Illegal PstI site found at 2715
Illegal PstI site found at 3015
Illegal PstI site found at 3555 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 655
Illegal BglII site found at 1217
Illegal BamHI site found at 4346
Illegal XhoI site found at 307
Illegal XhoI site found at 3729 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 655
Illegal PstI site found at 817
Illegal PstI site found at 2706
Illegal PstI site found at 2715
Illegal PstI site found at 3015
Illegal PstI site found at 3555 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 655
Illegal PstI site found at 817
Illegal PstI site found at 2706
Illegal PstI site found at 2715
Illegal PstI site found at 3015
Illegal PstI site found at 3555
Illegal NgoMIV site found at 287
Illegal AgeI site found at 2268
Illegal AgeI site found at 4401 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4172
Illegal BsaI.rc site found at 1792