Difference between revisions of "Part:BBa K3110041:Design"
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+ | iGEM ETH zurich 2015<br> | ||
+ | iGEM NUS Singapore 2016<br> |
Latest revision as of 15:57, 21 October 2019
lldRO1-J23117-lldRO2 Medium RBS sfGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 78
Illegal NheI site found at 101 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 175
Design Notes
None.
Source
sfGFP along with terminator was obtained from the Biobrick. lldRO1-J23117-lldRO2 amd B0032 was obtained as gBlock from IDT and these were joined together using SOEing (Spliced Overlap Extention) PCR.
References
iGEM ETH zurich 2015
iGEM NUS Singapore 2016