Profile

Name	GGGG-mCherry
Base pairs	1028
Molecular weight	28.5 kDa
Origin	synthetic, derived from Discosoma sp.
Parts	mCherry, GGGG-sequence, T7 Promoter, lac Operator, GASPAG Linker, Strep-Tag II, T7 Terminator
Properties	Red fluorescent, Ex λ: 587nm, Em λ: 610 nm

Usage and Biology

mCherry (BBa_K3187026) is a red fluorescent protein. Which is a synthetic protein derived from Discosoma sp. by directed evolution. The N-terminal GGGG-sequence (BBa_K3187018) can be fused to a protein with a C-terminal LPETGG-Sortase A link (BBa_K3187019) by Sortase A. We use mCherry as an easily imaged reporter for checking if the coupling worked.
The coding sequence was cloned in pET24 vector, containing the sequence of mCherry, a GGGG-sequence, a GASPAG Linker (BBa_K3187038), a Strep-Tag II (BBa_K3187025) for Purification, a T7 Promoter with lac Operator and an RBS (BBa_K3187029), a T7 Terminator (BBa_K3187032), a Start-Codon (BBa_J70593) and a Stop-Codon (BBa_K2868029). The coding sequence consists of 851 bp which are translated to 260 amino acids.^[1]

Results

For results regarding this part, please have a look at (BBa_K3187028)

Sequence and Features