Difference between revisions of "Part:BBa K3320016"

 
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<partinfo>BBa_K3320016 short</partinfo>
 
<partinfo>BBa_K3320016 short</partinfo>
  
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This is a part from our AND Gate, called hypoxia repressor unit. In the presence of hypoxia, SrpR is produced, repressing the Srp promoter with Tet repressor, allowing the production of GFPmut3 + HlyA
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This is a part from our AND Gate, called hypoxia repressor unit.  
  
  
  
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<!-- Add more about the biology of this part here
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<!-- Add more about the biology of this part here<!-- -->
 
===Usage and Biology===
 
===Usage and Biology===
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The hypoxia repressor unit is composed of the fdhF promoter, inducible in low concentrations of oxygen, expressing the Srp repressor protein, which represses the Srp promoter producing TetR that regulates the expression of BBa_K3320013 by Tet promoter.
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3320016 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3320016 SequenceAndFeatures</partinfo>

Revision as of 15:55, 21 October 2019


Hypoxia Repressor Unit

This is a part from our AND Gate, called hypoxia repressor unit.


Usage and Biology

The hypoxia repressor unit is composed of the fdhF promoter, inducible in low concentrations of oxygen, expressing the Srp repressor protein, which represses the Srp promoter producing TetR that regulates the expression of BBa_K3320013 by Tet promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 867
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 616
  • 1000
    COMPATIBLE WITH RFC[1000]