Difference between revisions of "Part:BBa K2950004"

 
 
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ferredoxinTOL reductase (TodA) is involved in the toluene dioxygenase pathway, the pathway we utilized to produce our aim product urushiol, and is identified in Pseudomonas putida. TodA is responsible for the transfer of two electrons from nicotinamide adenine dinucleotide (NADH) to Rieske-type ferredoxinTOL (TodB).
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<p>ferredoxinTOL reductase (TodA) is involved in the toluene dioxygenase pathway, the pathway we utilized to produce our aim product urushiol, and is identified in Pseudomonas putida. TodA is responsible for the transfer of two electrons from nicotinamide adenine dinucleotide (NADH) to Rieske-type ferredoxinTOL (TodB). TodA is 44 kDa, and is characterized via SDS-PAGE electrophoresis.</p>
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<img src="https://2019.igem.org/wiki/images/b/ba/T--BNDS_China--todA.jpeg" width=600px>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:55, 21 October 2019


ferredoxinTOL reductase (TodA)

ferredoxinTOL reductase (TodA) is involved in the toluene dioxygenase pathway, the pathway we utilized to produce our aim product urushiol, and is identified in Pseudomonas putida. TodA is responsible for the transfer of two electrons from nicotinamide adenine dinucleotide (NADH) to Rieske-type ferredoxinTOL (TodB). TodA is 44 kDa, and is characterized via SDS-PAGE electrophoresis.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1173
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 226
    Illegal AgeI site found at 319
  • 1000
    COMPATIBLE WITH RFC[1000]