Difference between revisions of "Part:BBa K2908676"
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The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of GAD and miR101-BS for sequence-specific expression of GAD.Therefore,we used our p(GATA3)p(BBa_K2908000) as a specific promoter. At the same time we used our miR101-BD(BBa_K2908668) to bind different concentrations of miR101 and fuse it to 3' end so that can regulate the transactivation domain of GAD.In this way we can ensure the specificity and independence of this protein. | The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of GAD and miR101-BS for sequence-specific expression of GAD.Therefore,we used our p(GATA3)p(BBa_K2908000) as a specific promoter. At the same time we used our miR101-BD(BBa_K2908668) to bind different concentrations of miR101 and fuse it to 3' end so that can regulate the transactivation domain of GAD.In this way we can ensure the specificity and independence of this protein. | ||
| − | + | New part: s(GATA3)p-GAD-miR101-BD (BBa_K2908676) [GAD is totally the same as Gal4-VP16] | |
https://2019.igem.org/wiki/images/c/c8/T--CSU_CHINA--2908676-1.jpg<br> | https://2019.igem.org/wiki/images/c/c8/T--CSU_CHINA--2908676-1.jpg<br> | ||
The part improved in the specificity in Triple negative cancer (TNBC). | The part improved in the specificity in Triple negative cancer (TNBC). | ||
| − | + | Existing part: CMV-Gal4-VP16 (BBa_K2580666) | |
https://2019.igem.org/wiki/images/5/53/T--CSU_CHINA--2908676-2.jpg<br> | https://2019.igem.org/wiki/images/5/53/T--CSU_CHINA--2908676-2.jpg<br> | ||
The part has low specificity in the TNBC cells compared to normal cells although the team members who created the part stated that it is a cancer-specific part. | The part has low specificity in the TNBC cells compared to normal cells although the team members who created the part stated that it is a cancer-specific part. | ||
https://2019.igem.org/wiki/images/a/ab/T--CSU_CHINA--2908676-3.jpg<br> | https://2019.igem.org/wiki/images/a/ab/T--CSU_CHINA--2908676-3.jpg<br> | ||
| + | As for this new part we created this year, it contains the brand new synthetic promoter we designed (BBa_K2908000), the existing sequence in the previous iGEM parts, GAD (Gal4-VP16), and the new designed part miR101-BD. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 15:53, 21 October 2019
s(GATA3)p-GAD-miR101-BS
The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of GAD and miR101-BS for sequence-specific expression of GAD.Therefore,we used our p(GATA3)p(BBa_K2908000) as a specific promoter. At the same time we used our miR101-BD(BBa_K2908668) to bind different concentrations of miR101 and fuse it to 3' end so that can regulate the transactivation domain of GAD.In this way we can ensure the specificity and independence of this protein.
New part: s(GATA3)p-GAD-miR101-BD (BBa_K2908676) [GAD is totally the same as Gal4-VP16]
The part improved in the specificity in Triple negative cancer (TNBC).
Existing part: CMV-Gal4-VP16 (BBa_K2580666)
The part has low specificity in the TNBC cells compared to normal cells although the team members who created the part stated that it is a cancer-specific part.
As for this new part we created this year, it contains the brand new synthetic promoter we designed (BBa_K2908000), the existing sequence in the previous iGEM parts, GAD (Gal4-VP16), and the new designed part miR101-BD.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 99
Illegal NheI site found at 584 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 360
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 279