Difference between revisions of "Part:BBa K3110021:Design"
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===Source=== | ===Source=== | ||
| − | + | Weak promoter -Weak RBS-lldR flank and lldD flank-terminator were synthesized by IDT <br> | |
lldR+lldD was amplified from the genome | lldR+lldD was amplified from the genome | ||
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| − | |||
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===References=== | ===References=== | ||
Revision as of 15:51, 21 October 2019
Weak Promoter Weak RBS lldR+lldD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1987
Illegal AgeI site found at 619 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1424
Design Notes
Source
Weak promoter -Weak RBS-lldR flank and lldD flank-terminator were synthesized by IDT
lldR+lldD was amplified from the genome
References
iGEM ETH zurich 2015
iGEM NUS Singapore 2016
Aguilera, L., Campos, E., Gimenez, R., Badia, J., Aguilar, J., & Baldoma, L. (2008). Dual Role of LldR in Regulation of the lldPRD Operon, Involved in L-Lactate Metabolism in Escherichia coli. Journal of Bacteriology, 190(8), 2997–3005. doi:10.1128/jb.02013-07