Difference between revisions of "Part:BBa K3132003"

Latest revision as of 15:50, 21 October 2019

PIP-VP64

This part is transformed from the part: ZF_PIP_KRAB(TF-KRAB-2) (BBa_K2446045) of iGEM17_Fudan. It is a synthetic transcription factor (SynTF). It contains three core domains from N-terminal to C-terminal: PIP DNA binding domain (DBD), nuclear location sequence and transcription regulating domain. To turn this part from an inhibitory one to an activating one, we use VP64 to displace the KRAB gene. So, we use PIP-VP64 as a specific transcription factor to activate the expression of our downstream synthetic promote.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 475

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3132003 short</partinfo>
-This part is transformed from the part: ZF_PIP_KRAB(TF-KRAB-2) (BBa_K2446045) of iGEM17_Fudan. It is a synthetic transcription factor (SynTF). It contains three core domains from N-terminal to C-terminal: PIP DNA binding domain (DBD), nuclear location sequence and transcription regulating domain. To turn this part from an inhibitory one to an activating one, we use VP64 to displace the KRAB gene.
+This part is transformed from the part: ZF_PIP_KRAB(TF-KRAB-2) (<partinfo>BBa_K2446045</partinfo>) of iGEM17_Fudan. It is a synthetic transcription factor (SynTF). It contains three core domains from N-terminal to C-terminal: PIP DNA binding domain (DBD), nuclear location sequence and transcription regulating domain. To turn this part from an inhibitory one to an activating one, we use VP64 to displace the KRAB gene.
 So, we use PIP-VP64 as a specific transcription factor to activate the expression of our downstream synthetic promote.