Difference between revisions of "Part:BBa K3139014:Experience"
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===Applications of BBa_K3139014=== | ===Applications of BBa_K3139014=== | ||
| + | ===Background=== | ||
| + | Composite part BBa_K3139014, J23100-fusion protein-B0015-J23106-TEVp-TEVp site-HasA-B0010, anti-Plasmodium system. We choose this part as the best composite part, because its function was the most superior among all the parts of the same type (Figure.1 Lane 3). It also provided significant data for our mathematic model which simulated different promoter combinations (shown in Figure.2) of anti-Plasmodium system and predicted the possibly most superior combination. | ||
| + | the amount of both TEV protease and anti-Plasmodium fusion protein should be kept at a certain proportion to avoid a waste of cell resources. | ||
| + | If the TEV protease is excessive, it shall be wasted though the fusion proteins are cleaved completely. If the fusion protein is excessive, it cannot be cleaved completely with insufficient TEV protease. | ||
| + | <src="T--NAU-CHINA--promoter.png"><src="T--NAU-CHINA--promotercombination.png"> | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 15:31, 21 October 2019
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K3139014
Background
Composite part BBa_K3139014, J23100-fusion protein-B0015-J23106-TEVp-TEVp site-HasA-B0010, anti-Plasmodium system. We choose this part as the best composite part, because its function was the most superior among all the parts of the same type (Figure.1 Lane 3). It also provided significant data for our mathematic model which simulated different promoter combinations (shown in Figure.2) of anti-Plasmodium system and predicted the possibly most superior combination. the amount of both TEV protease and anti-Plasmodium fusion protein should be kept at a certain proportion to avoid a waste of cell resources. If the TEV protease is excessive, it shall be wasted though the fusion proteins are cleaved completely. If the fusion protein is excessive, it cannot be cleaved completely with insufficient TEV protease. <src="T--NAU-CHINA--promoter.png"><src="T--NAU-CHINA--promotercombination.png">
User Reviews
UNIQ43494cbf610cf2e4-partinfo-00000000-QINU UNIQ43494cbf610cf2e4-partinfo-00000001-QINU