Difference between revisions of "Part:BBa K3038006:Design"

 
 
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all the coding sequences have been designed with the RFC10 standard and optimized for the GC content.
 
all the coding sequences have been designed with the RFC10 standard and optimized for the GC content.
  
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A 6 his Tag has been added at the Cterm part of the TesA enzyme  
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<center>https://static.igem.org/mediawiki/parts/1/11/T--Poitiers--TDesignTesAMlut11700.png<br>
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A <cmyc tag has been added at the Nterm part of the Mlut enzyme  
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<strong>Design of the TesA/Mlut_11700 gene with the Geneious Software.</strong><br>The design show restriction enzymes like EcoRI, XbaI, SpeI and PstI. The gene includes pBAD promoter and its terminater. Finally 6-His and C-myc tag are present in TesA and Mlut_11700.</center><br>
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A 6 his Tag has been added at the Cterm part of the TesA enzyme and a c-cmyc tag has been added at the Nterm part of the Mlut enzyme.
  
  
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===References===
 
===References===
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Latest revision as of 15:31, 21 October 2019


PBAD--TesA-CTer-6His--Mlut-Nter-Cmyc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 3474
    Illegal XhoI site found at 3717
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3608
    Illegal BsaI.rc site found at 3839
    Illegal SapI site found at 961
    Illegal SapI site found at 1964


Design Notes

all the coding sequences have been designed with the RFC10 standard and optimized for the GC content.

T--Poitiers--TDesignTesAMlut11700.png
Design of the TesA/Mlut_11700 gene with the Geneious Software.
The design show restriction enzymes like EcoRI, XbaI, SpeI and PstI. The gene includes pBAD promoter and its terminater. Finally 6-His and C-myc tag are present in TesA and Mlut_11700.

A 6 his Tag has been added at the Cterm part of the TesA enzyme and a c-cmyc tag has been added at the Nterm part of the Mlut enzyme.


Source

cloning of the different part using the Biobrick design and classic cloning techniques

References