Difference between revisions of "Part:BBa K3259003"
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<partinfo>BBa_K3259003 short</partinfo> | <partinfo>BBa_K3259003 short</partinfo> | ||
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This construct was designed to quantify BBa_K325901. | This construct was designed to quantify BBa_K325901. | ||
Revision as of 15:29, 21 October 2019
Contructs for characterization of pCold-Rhl hybrid promoter
This construct was designed to quantify BBa_K325901. BBa_K3259004 is expected to be expressed by adding C4-HSL at low temperature condition.
This construct was made by combining GFP-containing part located at the downstream of BBa_K3259001 and other part that constitutively produces RhlR.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1079
Illegal NheI site found at 1102 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1373
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1848
Illegal BsaI.rc site found at 988