Difference between revisions of "Part:BBa K3259002"

 
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<partinfo>BBa_K3259002 short</partinfo>
 
<partinfo>BBa_K3259002 short</partinfo>
  
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This part was made into a promoter with two control methods by binding the RhlR / PAI2 operator to a blue light sensitive promoter.
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This construct was designed to quantify BBa_K3259000.
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BBa_K3259000 is expected to be induced when both blue light and RhlR /PAI1 complex are present.
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This construct was created by combining parts with GFP downstream of BBa_K3259000 and parts that constitutively produce RhlR.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:25, 21 October 2019


Contructs for characterization of pYcgZ-Rhl hybrid promoter

This construct was designed to quantify BBa_K3259000. BBa_K3259000 is expected to be induced when both blue light and RhlR /PAI1 complex are present.

This construct was created by combining parts with GFP downstream of BBa_K3259000 and parts that constitutively produce RhlR.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 899
    Illegal NheI site found at 922
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1193
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1668
    Illegal BsaI.rc site found at 808