Difference between revisions of "Part:BBa K3183001"

Revision as of 15:10, 21 October 2019

Surface (S)-Layer Protein Constitutive Promoter for Lactobacillus sp.

P-slp is a constitutive promoter which can be used in Lactobacillus reuteri 10023C, and may have uses in other Lactobacillus species.

This part encodes the promoter of the surface level protein gene in lactic acid bacteria. The (S)-layer protein (slp) promoter is derived from Lactobacillus acidophilus, and is responsible for the high level of transcription of stable mRNAs coding the S-protein monomers (Boot & Pouwels, 1996; Boot et al., 1996). It was characterised in six strains of Lactobacillus reuteri and Lactococcus lactis spp. cremoris MG1363 by Lizier et al, 2010. The promoter shows good activity in L. lactis spp. cremoris MG1363, but has very little activity in L. reuteri.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Characterisation

This part forms the composite part BBa_K3183102. Data for this part is shown below.

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-The (S)-layer protein (slp) promoter is derived from <i>Lactobacillus acidophilus</i>, and is responsible for the high level of transcription of stable mRNAs coding the S-protein monomers (Boot & Pouwels, 1996; Boot et al., 1996). It was characterised in six strains of <i>Lactobacillus reuteri</i> and <i>Lactococcus lactis </i>spp.<i> cremoris</i> MG1363 by <a href="https://academic.oup.com/femsle/article/308/1/8/513999">Lizier et al, 2010.</a>
+This part encodes the promoter of the surface level protein gene in lactic acid bacteria.
+The (S)-layer protein (slp) promoter is derived from <i>Lactobacillus acidophilus</i>, and is responsible for the high level of transcription of stable mRNAs coding the S-protein monomers (Boot & Pouwels, 1996; Boot et al., 1996). It was characterised in six strains of <i>Lactobacillus reuteri</i> and <i>Lactococcus lactis </i>spp.<i> cremoris</i> MG1363 by <a href="https://academic.oup.com/femsle/article/308/1/8/513999">Lizier et al, 2010.</a> The promoter shows good activity in L. lactis spp. cremoris MG1363, but has very little activity in L. reuteri.
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