Difference between revisions of "Part:BBa J10052:Experience"
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| − | <i>Figure 1. The highlighted points in yellow represent BBa_J10052 (weak promoter/ medium RBS) compared to BBa_J10050(weak promoter/ weak RBS) and BBa_J10051(weak promoter/ strong RBS), both in light blue. The weak, medium, and strong RBS have similar expression levels when in combination with the weak promoter. </i> | + | <center><i>Figure 1. The highlighted points in yellow represent BBa_J10052 (weak promoter/ medium RBS) compared to BBa_J10050(weak promoter/ weak RBS) and BBa_J10051(weak promoter/ strong RBS), both in light blue. The weak, medium, and strong RBS have similar expression levels when in combination with the weak promoter. </i></center> |
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Revision as of 15:08, 21 October 2019
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_J10052
User Reviews
Lambert_GA 2019 Characterization
Characterization of weak promoter (BBa_J23113) and weak RBS (BBa_B0031)
Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10052 would result in the least amount of expression out of the tested combinations, but this part, along with BBa_J10050 and BBa_J10051, all shared the lowest expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths.
UNIQ5f8127de8a498970-partinfo-00000000-QINU
UNIQ5f8127de8a498970-partinfo-00000001-QINU