Difference between revisions of "Part:BBa K2992002"

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===Characterisation===
 
===Characterisation===
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We made three <i>BotR</i>-expression modules comprising of the <i>BotR</i> sigma factor gene under the control of the native <i>BotR</i> promotor,an induible system and one with no promotor. See the [https://2019.igem.org/Team:Nottingham/Results results page] for more information  <br><br><br>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 15:07, 21 October 2019


botR gene from C. botulinum

botR encodes a positive transcriptional regulator of C. botulinum neurotoxin production which functions as an alternative sigma factor.

Usage and Biology

BotR regulates the the expression of boutlinum neruotoxin and non-toxic associated proteins during the late exponential and early stationary growth phases. We have integrated botR into the genome of C. sporogenes to generate a reporter strain to validate the concept of using volatile reporters to predict the production of botulinum neurotoxin following food manufacturing processes.

Characterisation

We made three BotR-expression modules comprising of the BotR sigma factor gene under the control of the native BotR promotor,an induible system and one with no promotor. See the results page for more information



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Raffestin, S., Dupuy, B., Marvaud, J. and Popoff, M. (2004). BotR/A and TetR are alternative RNA polymerase sigma factors controlling the expression of the neurotoxin and associated protein genes in Clostridium botulinum type A and Clostridium tetani. Molecular Microbiology, 55(1), pp.235-249.

Zhang, Z., Korkeala, H., Dahlsten, E., Sahala, E., Heap, J., Minton, N. and Lindström, M. (2013). Two-Component Signal Transduction System CBO0787/CBO0786 Represses Transcription from Botulinum Neurotoxin Promoters in Clostridium botulinum ATCC 3502. PLoS Pathogens, 9(3), p.e1003252.