Difference between revisions of "Part:BBa K3170999"
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We constructed a FRET system for the detection of TEVp by the CFP-target sequence-YFP. When TEVp was expressed, the FRET system showed fluorescence transfer; when TEVp was not expressed, there was no fluorescence transfer. Finally, the fluorescent color expressed by the FRET system can judge the activity of TEVp. | We constructed a FRET system for the detection of TEVp by the CFP-target sequence-YFP. When TEVp was expressed, the FRET system showed fluorescence transfer; when TEVp was not expressed, there was no fluorescence transfer. Finally, the fluorescent color expressed by the FRET system can judge the activity of TEVp. | ||
| + | ==Experimental Results== | ||
| + | [[File:T--LZU-CHINA--Ras.png|600px|thumb|center|]] | ||
| + | [[File:T--LZU-CHINA--Flu.png|600px|thumb|center|]] | ||
| + | <ul> | ||
| + | <li>Imaging begins approximately 2 hours after the media is replaced with a fluorescent imaging medium. Get images every 1 hour. SW1990 cells were observed using 440 nm excitation light, and the cells turned from cyan to yellow.</li> | ||
| + | </ul> | ||
| + | ===Usage and Biology=== | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 15:01, 21 October 2019
FRET system for detecting TEVp activity
We constructed a FRET system for the detection of TEVp by the CFP-target sequence-YFP. When TEVp was expressed, the FRET system showed fluorescence transfer; when TEVp was not expressed, there was no fluorescence transfer. Finally, the fluorescent color expressed by the FRET system can judge the activity of TEVp.
Experimental Results
- Imaging begins approximately 2 hours after the media is replaced with a fluorescent imaging medium. Get images every 1 hour. SW1990 cells were observed using 440 nm excitation light, and the cells turned from cyan to yellow.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 694
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]