Difference between revisions of "Part:BBa J10053:Experience"
| Line 9: | Line 9: | ||
Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10053 would result in a moderate amount of expression out of the tested combinations, but this part, along with BBa_J10054 and BBa_J10055, all shared moderate expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths. | Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10053 would result in a moderate amount of expression out of the tested combinations, but this part, along with BBa_J10054 and BBa_J10055, all shared moderate expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths. | ||
| − | |||
[[File:T--Lambert GA--tuning4b.png|800px]] | [[File:T--Lambert GA--tuning4b.png|800px]] | ||
| + | [[File:T--Lambert GA--tuning4a.png|800px]] | ||
<!-- DON'T DELETE --><partinfo>BBa_J10053 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_J10053 StartReviews</partinfo> | ||
Revision as of 14:52, 21 October 2019
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J10053
User Reviews
Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10053 would result in a moderate amount of expression out of the tested combinations, but this part, along with BBa_J10054 and BBa_J10055, all shared moderate expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths.
UNIQ74309f2bac3d5839-partinfo-00000000-QINU UNIQ74309f2bac3d5839-partinfo-00000001-QINU