Difference between revisions of "Part:BBa J10053:Experience"
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Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10053 would result in a moderate amount of expression out of the tested combinations, but this part, along with BBa_J10054 and BBa_J10055, all shared moderate expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths. | Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10053 would result in a moderate amount of expression out of the tested combinations, but this part, along with BBa_J10054 and BBa_J10055, all shared moderate expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths. | ||
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[[File:T--Lambert GA--tuning4b.png|800px]] | [[File:T--Lambert GA--tuning4b.png|800px]] | ||
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Revision as of 14:52, 21 October 2019
This experience page is provided so that any user may enter their experience using this part.
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Applications of BBa_J10053
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Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10053 would result in a moderate amount of expression out of the tested combinations, but this part, along with BBa_J10054 and BBa_J10055, all shared moderate expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths.
UNIQd589893a1a932d54-partinfo-00000000-QINU UNIQd589893a1a932d54-partinfo-00000001-QINU