Difference between revisions of "Part:BBa K3320005"
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pPhlF is an orthogonal promoter repressed by PhlF Repressor. In the absence of PhlF repressor, produced by the lactate promoter, this part is constitutively expressing TetR, which represses the production of BBa_K3320013. | pPhlF is an orthogonal promoter repressed by PhlF Repressor. In the absence of PhlF repressor, produced by the lactate promoter, this part is constitutively expressing TetR, which represses the production of BBa_K3320013. | ||
===Assembly=== | ===Assembly=== | ||
| − | This part was cloned on pUC19 vector, totaling 4587 bp. | + | <p>This part was cloned on pUC19 vector, totaling 4587 bp. </p> |
| − | [[File:BBa_K3320005_Map.png]] | + | [[File:BBa_K3320005_Map.png|500px|]] |
| + | <p> Figure 1. BBa_K332005 plasmidial map. | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3320005 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3320005 SequenceAndFeatures</partinfo> | ||
Revision as of 14:49, 21 October 2019
Basic Repressor Unit
This composite part is the basic constitution of our AND gate, repressing pTet with TetR protein regulated by phiF promoter.
Usage and Biology
pPhlF is an orthogonal promoter repressed by PhlF Repressor. In the absence of PhlF repressor, produced by the lactate promoter, this part is constitutively expressing TetR, which represses the production of BBa_K3320013.
Assembly
This part was cloned on pUC19 vector, totaling 4587 bp.
Figure 1. BBa_K332005 plasmidial map. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1596