Difference between revisions of "Part:BBa J10052:Experience"
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===User Reviews=== | ===User Reviews=== | ||
Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10052 would result in the least amount of expression out of the tested combinations, but this part, along with BBa_J10050 and BBa_J10051, all shared the lowest expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths. | Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10052 would result in the least amount of expression out of the tested combinations, but this part, along with BBa_J10050 and BBa_J10051, all shared the lowest expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths. | ||
| + | = Lambert_GA 2019 Characterization = | ||
| + | [[File:T--Lambert GA--tuning2b.png|800px]] | ||
| + | [[File:T--Lambert GA--tuning2a.png|800px]] | ||
| + | |||
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Revision as of 14:41, 21 October 2019
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J10052
User Reviews
Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10052 would result in the least amount of expression out of the tested combinations, but this part, along with BBa_J10050 and BBa_J10051, all shared the lowest expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths.
Lambert_GA 2019 Characterization
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