Difference between revisions of "Part:BBa K2924052:Design"

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===Source===
 
===Source===
  
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PBSMul1-SPNprE plasmid was kindly provided by Dr. Andreas Knapp from the research center Jülich. The DNA sequence was acquired by reverse-translating the protein sequence of a-s2-casein. a-s2-casein was commercial synthesized by GenScript.
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PBSMul1-SPNprE plasmid was kindly provided by Dr. Andreas Knapp from the research center Jülich. The DNA sequence was acquired by reverse-translating the protein sequence of α-s2-casein. <i>α-s2-casein</i> was commercial synthesized by GenScript.
  
 
===References===
 
===References===

Revision as of 14:38, 21 October 2019


Hpall + SPNprE + alpha s2 + fd terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1124
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 554


Design Notes

Expression system for the expression and secretion of the protein in B. subtilis

Source

PBSMul1-SPNprE plasmid was kindly provided by Dr. Andreas Knapp from the research center Jülich. The DNA sequence was acquired by reverse-translating the protein sequence of α-s2-casein. α-s2-casein was commercial synthesized by GenScript.

References

Brockmeier, U., et al. (2006). "Systematic screening of all signal peptides from Bacillus subtilis: a powerful strategy in optimizing heterologous protein secretion in Gram-positive bacteria." J Mol Biol 362(3): 393-402.

Knapp, A., et al. (2017). "Activity-independent screening of secreted proteins using split GFP." J Biotechnol 258: 110-116.