Difference between revisions of "Part:BBa K2999001"
 
Line 3: Line 3:
 
<partinfo>BBa_K2999001 short</partinfo>
 
<partinfo>BBa_K2999001 short</partinfo>
  
−
This part is mainly used for the encoding extracellular polysaccharide hydrolase PslG and the hydrolysis of such extracellular polysaccharides of ''Pseudomonas aeruginosa'' PAO1 biofilm . The first 50 bp of the sequence is its ribosome binding site.
+
This part is mainly used to encode the extracellular polysaccharide hydrolase PslG and the hydrolysis of such extracellular polysaccharides of biofilm produced by ''Pseudomonas aeruginosa'' PAO1 . The first 50 bp of the sequence is its ribosome binding site.
 
For compatibility, 254<sup>th</sup> nucleotide G was changed to A (same as leucine) and 1358<sup>th</sup> nucleotide G to A (same as leucine).
 
For compatibility, 254<sup>th</sup> nucleotide G was changed to A (same as leucine) and 1358<sup>th</sup> nucleotide G to A (same as leucine).
  

Latest revision as of 14:32, 21 October 2019


RBS+PslG

This part is mainly used to encode the extracellular polysaccharide hydrolase PslG and the hydrolysis of such extracellular polysaccharides of biofilm produced by Pseudomonas aeruginosa PAO1 . The first 50 bp of the sequence is its ribosome binding site. For compatibility, 254th nucleotide G was changed to A (same as leucine) and 1358th nucleotide G to A (same as leucine).

Usage and Biology

Please click:BBa_K2999009


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 356
    Illegal XhoI site found at 360
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 660
    Illegal NgoMIV site found at 849
    Illegal NgoMIV site found at 857
  • 1000
    COMPATIBLE WITH RFC[1000]