Difference between revisions of "Part:BBa K2999000"
 
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<partinfo>BBa_K2999000 short</partinfo>
 
<partinfo>BBa_K2999000 short</partinfo>
  
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This part is mainly used to encode the extracellular polysaccharide hydrolase PelA of ''Pseudomonas aeruginosa'' PAO1 biofilm and its hydrolysis. The first 100 bp of the sequence is its own RBS region.
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This part is mainly used to encode the extracellular polysaccharide hydrolase PelA and its hydrolysis of biofilm produced by ''Pseudomonas aeruginosa'' PAO1 . The first 100 bp of the sequence is its own RBS region.
  
  

Latest revision as of 14:27, 21 October 2019


RBS+PelA

This part is mainly used to encode the extracellular polysaccharide hydrolase PelA and its hydrolysis of biofilm produced by Pseudomonas aeruginosa PAO1 . The first 100 bp of the sequence is its own RBS region.


Usage and Biology

Please click:BBa_K2999009

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2427
    Illegal BamHI site found at 1410
    Illegal BamHI site found at 1575
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 191
    Illegal NgoMIV site found at 215
    Illegal NgoMIV site found at 236
    Illegal NgoMIV site found at 337
    Illegal NgoMIV site found at 563
    Illegal NgoMIV site found at 1271
    Illegal NgoMIV site found at 1320
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1157
    Illegal BsaI site found at 1783
    Illegal BsaI.rc site found at 1358