Difference between revisions of "Part:BBa K3117006:Design"
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===Design Notes===
 
===Design Notes===
 
We considered which leader sequences are most effective to effect secretion of our protein of interest.
 
We considered which leader sequences are most effective to effect secretion of our protein of interest.
 +
The Igk leader sequence was codon optimized for CHO cells.
  
  

Revision as of 14:05, 21 October 2019


Igk leader sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We considered which leader sequences are most effective to effect secretion of our protein of interest. The Igk leader sequence was codon optimized for CHO cells.


Source

This part comes from genomic sequence.

References

Feng, Y., Gong, R., & Dimitrov, D. S. (2011). Design, expression and characterization of a soluble single-chain functional human neonatal Fc receptor. Protein expression and purification, 79(1), 66-71.