Difference between revisions of "Part:BBa K3117006:Experience"
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===Applications of BBa_K3117006=== | ===Applications of BBa_K3117006=== | ||
| + | In our project the Igk leader was added to three proteins (BBa_K3117026, BBa_K3117030, BBa_K3117029) for the transport of the protein into the medium, after transfection into HEK 293T cells. | ||
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| + | Bild Ernte | ||
| + | Figure 1: Western blot of the harvest after transfection into HEK 293T cells with an anti-His-Tag antibody | ||
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| + | In Fig. 1 the western blot of the harvest of HEK 293T cells can be seen. Those proteins were detected via their His-Tag. The presence of the proteins in the medium shows, that the Igk leader was able to direct all three proteins into the secretory way. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 14:03, 21 October 2019
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K3117006
In our project the Igk leader was added to three proteins (BBa_K3117026, BBa_K3117030, BBa_K3117029) for the transport of the protein into the medium, after transfection into HEK 293T cells.
Bild Ernte Figure 1: Western blot of the harvest after transfection into HEK 293T cells with an anti-His-Tag antibody
In Fig. 1 the western blot of the harvest of HEK 293T cells can be seen. Those proteins were detected via their His-Tag. The presence of the proteins in the medium shows, that the Igk leader was able to direct all three proteins into the secretory way.
User Reviews
UNIQ9bd4f7883f8e2cc4-partinfo-00000000-QINU UNIQ9bd4f7883f8e2cc4-partinfo-00000001-QINU