Difference between revisions of "Part:BBa K3093006"
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Latest revision as of 13:50, 21 October 2019
His tag-cenA-hlyA
Usage and Biology
Cellulomonas fimi cenA gene with a his tag and a hlyA tag.
Cellulomonas fimi cenA gene encoding endoglucanase.They expressed carboxymethylcellulase activity in Escherichia coli.
For more info visit https://www.ncbi.nlm.nih.gov/nuccore/M15823.1
And this part is used in the BBa_K3093008
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 88
Illegal NotI site found at 1232 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1145
Illegal BamHI site found at 36
Illegal BamHI site found at 281
Illegal XhoI site found at 45
Illegal XhoI site found at 643
Illegal XhoI site found at 892 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 423
Illegal NgoMIV site found at 1348 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 327