Difference between revisions of "Part:BBa K3093007"
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For more info visit https://www.ncbi.nlm.nih.gov/nuccore/L11080.1 | For more info visit https://www.ncbi.nlm.nih.gov/nuccore/L11080.1 | ||
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Latest revision as of 13:50, 21 October 2019
His tag-cex-hlyA
Usage and Biology
Cellulomonas fimi exo-beta-1,4-glucanase and beta-1,4-xylanase gene（cex) with a his tag and a hlyA tag.
In Cellulomonas fimi the cex gene encodes an exoglucanase (Exg) involved in the degradation of cellulose.The codon usage in C. fimi appears to be quite different from that of Escherichia coli. A dramatic (98.5%) bias occurs for G or C in the third position for the 35 codons utilized in the cex gene.
For more info visit https://www.ncbi.nlm.nih.gov/nuccore/L11080.1
And this part is used in the BBa_K3093009
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 578
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 36
Illegal XhoI site found at 45 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 211
Illegal NgoMIV site found at 584
Illegal NgoMIV site found at 1086 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 631
Illegal SapI.rc site found at 714