Difference between revisions of "Part:BBa K2999001"

Revision as of 13:43, 21 October 2019

RBS+PslG

This part is mainly used for the extracellular polysaccharide hydrolase PslG encoding Pseudomonas aeruginosa PAO1 biofilm and the hydrolysis of such extracellular polysaccharides. The first 50 bp of the sequence is its ribosome binding site. For compatibility, 254^{th^{nucleotide G was changed to A (same as leucine) and 1358 nucleotide G to A (same as leucine).}}

Usage and Biology

Please click:BBa_K2999009

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 356
Illegal XhoI site found at 360
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 660
Illegal NgoMIV site found at 849
Illegal NgoMIV site found at 857
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2999001 short</partinfo>
-This part is mainly used for the extracellular polysaccharide hydrolase PslG encoding ''Pseudomonas aeruginosa'' PAO1 biofilm and the hydrolysis of such extracellular polysaccharides. The first 50 BP of the sequence is its ribosome binding site.
+This part is mainly used for the extracellular polysaccharide hydrolase PslG encoding ''Pseudomonas aeruginosa'' PAO1 biofilm and the hydrolysis of such extracellular polysaccharides. The first 50 bp of the sequence is its ribosome binding site.
-For compatibility, 254<sup>th<sup> nucleotide G was changed to A (same as leucine) and 1358 nucleotide G to A (same as leucine).
+For compatibility, 254<sup>th<sup><br/> nucleotide G was changed to A (same as leucine) and 1358 nucleotide G to A (same as leucine).
 ===Usage and Biology===