Difference between revisions of "Part:BBa K2974410:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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The results for the trigger were confirmed through a dual plasmid transformation in BL21 <i>E. coli.</i> The dual plasmid transformation presented greater fluorescence measured in lux values in comparison to the single trigger transformation, confirming the function of the dual toehold. The dual plasmid transformation would not succeed without the correct <i>C. elegans</i> trigger intermediate, so the function of this part (BBa_K2974410) has been validated through experimentation and sequencing (see BBa_K2974400 for sequencing confirmation).  
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how you used this part and how it worked out.
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<center>[[File:T--Lambert GA--FLUOROCHART.png|500px]]</center>
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<center><I>Figure 1: This graph compares the mean lux values of the C. elegans toehold switch, trigger, and the dual plasmid. The toehold switch and trigger have very similar lux values to LB and plain cells, whereas the dual plasmid has a higher lux value, indicating fluorescence.</I></center>
 
===Applications of BBa_K2974410===
 
===Applications of BBa_K2974410===
  

Revision as of 13:40, 21 October 2019


The results for the trigger were confirmed through a dual plasmid transformation in BL21 E. coli. The dual plasmid transformation presented greater fluorescence measured in lux values in comparison to the single trigger transformation, confirming the function of the dual toehold. The dual plasmid transformation would not succeed without the correct C. elegans trigger intermediate, so the function of this part (BBa_K2974410) has been validated through experimentation and sequencing (see BBa_K2974400 for sequencing confirmation).

T--Lambert GA--FLUOROCHART.png


Figure 1: This graph compares the mean lux values of the C. elegans toehold switch, trigger, and the dual plasmid. The toehold switch and trigger have very similar lux values to LB and plain cells, whereas the dual plasmid has a higher lux value, indicating fluorescence.

Applications of BBa_K2974410

User Reviews

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