Difference between revisions of "Part:BBa K2992019"
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===References=== | ===References=== | ||
| − | + | RiboCas: A Universal CRISPR-Based Editing Tool for Clostridium. (2019). ACS Synthetic Biology, [online] p. Available at: https://pubs.acs.org/doi/abs/10.1021/acssynbio.9b00075 [Accessed 21 Oct. 2019]. | |
| − | Hartman and Melville | + | |
| + | Minton, N., Ehsaan, M., Humphreys, C., Little, G., Baker, J., Henstra, A., Liew, F., Kelly, M., Sheng, L., Schwarz, K. and Zhang, Y. (2016). A roadmap for gene system development in Clostridium. Anaerobe, 41, pp.104-112. 2019 RiboCas - update | ||
| + | |||
| + | Hartman, A., Liu, H. and Melville, S. (2010). Construction and Characterization of a Lactose-Inducible Promoter System for Controlled Gene Expression inClostridium perfringens. Applied and Environmental Microbiology, 77(2), pp.471-478. | ||
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Revision as of 13:38, 21 October 2019
bgaR gene from C. perfringens
Transcriptional regulator bgaR associated with a lactose inducible system from C. perfringens
Usage and Biology
BgaR is the transcriptional regulator associated with the BgaR-BgaL lactose inducible system found naturally on the genome of C. perfringens. Our group has recently utlised this regulatory system in order to generate a tightly regulate inducible system for CRISPR-Cas mutagenesis in the genus Clostridium (Cañadas et al., 2019). In our project, we use bgaR as part of the entire regulatory unit comprising bgaR, and its divergent promoter PbgaR -PbgaL with their associated 5’-UTR and RBS regions (hyperlinks and descriptions) to drive the expression of our volatile and FAST reporter genes in an inducible fashion. Doing so helps us fulfil our goal of generating reporter strains for the prediction of botulinum neurotoxin production following food manufacture.
Characterisation
Data incoming
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 368
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 368
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 368
Illegal BglII site found at 428 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 368
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 368
- 1000COMPATIBLE WITH RFC[1000]
References
RiboCas: A Universal CRISPR-Based Editing Tool for Clostridium. (2019). ACS Synthetic Biology, [online] p. Available at: https://pubs.acs.org/doi/abs/10.1021/acssynbio.9b00075 [Accessed 21 Oct. 2019].
Minton, N., Ehsaan, M., Humphreys, C., Little, G., Baker, J., Henstra, A., Liew, F., Kelly, M., Sheng, L., Schwarz, K. and Zhang, Y. (2016). A roadmap for gene system development in Clostridium. Anaerobe, 41, pp.104-112. 2019 RiboCas - update
Hartman, A., Liu, H. and Melville, S. (2010). Construction and Characterization of a Lactose-Inducible Promoter System for Controlled Gene Expression inClostridium perfringens. Applied and Environmental Microbiology, 77(2), pp.471-478.