Difference between revisions of "Part:BBa K3320019"

 
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<partinfo>BBa_K3320019 short</partinfo>
 
<partinfo>BBa_K3320019 short</partinfo>
  
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This is a part from our AND Gate, called lactate repressor unit. In the presence of lactate, the PhlF Repressor protein is expressed, repressing the PhlF promoter with Tet repressor, allowing the production of GFPmut3 + HlyA
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This is a part from our AND Gate, called lactate repressor unit.  
  
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<!-- Add more about the biology of this part here
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<!-- Add more about the biology of this part here <!-- -->
 
===Usage and Biology===
 
===Usage and Biology===
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In the presence of lactate, the PhlF Repressor protein is expressed, repressing the PhlF promoter with Tet repressor, allowing the production of GFPmut3 + HlyA
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3320019 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3320019 SequenceAndFeatures</partinfo>

Revision as of 13:36, 21 October 2019


Lactate Repressor Unit

This is a part from our AND Gate, called lactate repressor unit.

Usage and Biology

In the presence of lactate, the PhlF Repressor protein is expressed, repressing the PhlF promoter with Tet repressor, allowing the production of GFPmut3 + HlyA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 965
    Illegal NheI site found at 988
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 614
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3284